National Museum of the American Indian Commemorative Coin Act of 2000

National Museum of the American Indian Commemorative Coin Act of 2000, or American Buffalo Coin Commemorative Coin Act of 2000 - Directs the Secretary of the Treasury to mint and issue a maximum of 500,000 $1 dollar coins in commemoration of the opening of the Museum of the American Indian of the Smithsonian Institution.

Expresses the sense of Congress that the United States Mint Facility in Denver, Colorado, should strike such coins unless the Secretary determines that it would be technically or cost-prohibitive.

Mandates that the proceeds from sales surcharges be paid promptly to the National Museum of the American Indian of the Smithsonian Institution to: (1) commemorate the opening of the Museum; and (2)supplement the Museum's endowment and educational outreach funds.

Subjects the Museum to certain Federal audit requirements.

Instructs the Secretary to take actions to ensure that coin minting and issuance will not result in any net cost to the Government.