Public Transportation Systems Vulnerability Assessment and Reduction Act of 2005

Public Transportation Systems Vulnerability Assessment and Reduction Act of 2005 - Directs the Secretary of Homeland Security (Secretary) to: (1) review all government assessments conducted after September 11, 2001, of terrorist-related threats to all forms of public transportation, including related public gathering areas; (2) ensure that the assessments identify the critical assets of the system, including threats, and identify the extent to which backup systems ensure continued operation of the system in the event of an attack and the extent frontline transit employees have received training in security awareness, emergency preparedness, and response procedures; and (3) conduct, as necessary, additional assessments of vulnerabilities associated with any public transportation system.

Authorizes the Secretary to make grants to eligible transportation agencies for: (1) the training of frontline transit employees in emergency preparedness and response activities; and (2) the acquisition of approved equipment and technologies to assist in carrying them out. Requires allocation of grant amounts among eligible transportation agencies based on need for emergency preparedness and response training and equipment, earmarking at least ten percent of such amounts to eligible transportation agencies in non-urban areas.